Calcium Imaging

Nagiel, Aaron

Assistant Professor of Clinical Ophthalmology

The development and maintenance of specific synaptic connections between retinal neurons is critical to its function. Within the last 10 years it has become possible to grow 3-dimensional, multi-layered retinal organoids derived from human stem cells. This advance permits the study of human retinal development and the establishment of synaptic connectivity. Our goal is to elucidate mechanisms underlying synapse formation and specificity in the first synapse of the human visual system. Access to CRISPR-engineered organoids allows us to understand this process in the disease state.

Oghalai, John

Professor of Otolaryngology-Head and Neck Surgery and Viterbi School of Engineering, Biomedical Engineering

Our research is designed to better understand the fundamental changes in the inner ear that underlie progressive hearing loss and to develop novel techniques to treat this problem before it leads to a severe disability. We strive to understand the biological mechanisms of hearing loss and then translate this knowledge to directly and rapidly improve the care of patients with hearing loss.

Quadrato, Giorgia

Associate Professor of Stem Cell Biology and Regenerative Medicine

The Quadrato lab focuses on understanding the cellular and molecular basis of human brain development and mental disorders. We seek to produce meaningful work that advances the fundamental knowledge of our field and provides new tools to do it. By combining emerging models of the human brain with single-cell -omics approaches, we aim to identify brain region and cell type-specific disease mechanisms and, above all, new treatments for neuropsychiatric disorders. To improve the physiological relevance of human pluripotent stem cell-derived organoids, our lab is leveraging interdisciplinary strategies and technologies aimed at tighter regulatory control of organoid development through bioengineering approaches, along with newer unbiased organoid analysis readouts.

Schier, Lindsey

Associate Professor of Biological Sciences

The Schier lab seeks to understand how the chemical constituents of foods and fluids are sensed, how these oral and postoral signals are processed in the brain and channeled into the behavioral outputs that subserve energy balance.

Tao, Huizhong W.

Professor of Physiology and Neuroscience

My lab studies how the mouse brain processes visual information and transforms it into behavior. Our research focuses on identifying the neural circuits involved in visual perception and how these circuits drive visually guided actions. We use a combination of techniques—including electrophysiology to record neural activity, microendoscopic calcium imaging to monitor populations of neurons in freely moving animals, and both optogenetics and chemogenetics to precisely manipulate specific circuit components. By integrating these approaches, we aim to understand how visual signals are encoded, transmitted, and used to guide behavior at the level of individual neurons and larger networks.